Services

Custom Antibody Development/Production/Generation Services

WE provide a full range of custom antibody development/production services or the life science and biotech industry.

 

Custom Polyclonal Antibody Development/Production Service in Rabbit (2 New Zealand white rabbits)

Salient Features of Custom Polyclonal Antibody Development/Production Service

  • 12 Weeks protocol to produce antibody in 2 New Zealand White Rabbits (From confirmed Pure line Parental stock).
  • This will include 7 immunizations and 3 Production bleeds per animal.
  • Pre-immune sera of 5-10 ml per animal and a total of 30-40 ml of crude immune sera per rabbit.
  • *FREE ELISA Titer at first bleed against free immunogen.

Customer will be notified of immunization schedule and tentative date of production/Progress of the Project etc.

Immunogen required for custom polyclonal antibody development/production 

  1. For Protein (Provided by customer)-3 mg, Concentration: minimum 0.5 mg/ml
  2. For peptide (Min ~5-7 mg)

IMMUNIZATION AND BLEEDING SCHEDULE

WEEKS

PROCEDURE

BLEED/RABBIT

Week 1 (Pre-bleed)
Primary Immunization

Pre-Immune Sera and Primary Immunization

~5 ml*

Week 2 (Immunize)

2nd Immunization

 

Week 3 (Immunize)

3rd Immunization

 

Week 4 (Immunize)

4th Immunization

 

Week 5 (Immunize)

5th Immunization

 

Week 6 (Bleed-1)

1st production Bleed

~10 ml*

Week 7 (Immunize)

6th Immunization

 

Week 8 (Bleed-2)

2nd production Bleed

~10 ml*

Week 9 (Immunize)

7th Immunization

 

Week 10 (Bleed-3)

3rd production Bleed

~10 ml*

 

Extention of project per month (2 bleeds/Rabbit)

Bleed-1

~10 ml*

Bleed-2

~10 ml*

 

Terminal bleed
(1 bleed per Rabbit)

Bleed-1

~20-25 ml*

 

* In our custom polyclonal development/production service we usually provide the above mentioned sera from one rabbit/project but it may vary depending upon the health status of the animal.

The above schedule of custom polyclonal antibody development/production is approximate. We can also modify it according to the customer’s need.

Additional 4 weeks is required if the custom polyclonal antibody development/production service requires peptide synthesis, mass spec, KLH conjugation. For custom polyclonal antibody development service in which the immunogen is supplied can be started immediately. 

Purification:


Protein A : 15-20 mg of purified Antibody
Peptide affinity : 2-3 mg of purified Antibody

 

 

Additional Services

  • Custom peptide synthesis
  • Recombinant protein production for custom polyclonal antibody development/production in India
  • Custom polyclonal antibody development service against Phospho/Acetylated protein in India
  • Custom polyclonal antibody development service in Mice/Rat in India
  • Validation of custom polyclonal antibody for FACS, IP, IHC and IF
  • Custom monoclonal antibody development service in India

Terms and Conditions:
 

If the immunogen is peptide then it must contain a cysteine for conjugation.

  1. If the immunogen is already conjugated or it’s in gel, kindly provide us ~1 mg of free peptide/purified protein for ELISA.
  2. Recombinant protein and purified protein must be shipped in dry ice.
  3. Kindly provide antigen in soluble form.
  4. For peptide affinity purification customer should provide additional 6 mg peptide.
  5. Biostring Inc. guarantees ELISA positive sera for the immunogen synthesized.
  6. The titre of the antisera depends on the quality of the antigen.

 

Custom Monoclonal Antibody Development/Production Service

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We are a leading custom monoclonal antibody developing company. Availability of newer reagents paves the way for development of new diagnostic assays and helps in finding potential therapies for such diseases.

 

 

A. Partial Custom Monoclonal Antibody Development/Production Service 

 

For partial custom monoclonal antibody development/production service, customer provides immunogen in the form of purified protein, fusion protein, protein extracted from bacteria, virus, gel extracted protein, recombinant protein and synthetic peptide. We require a minimum of 5 mg of the protein at a minimum concentration of 0.5 mg/ml.  

B. Complete Custom Monoclonal Antibody Development/Production Service-Peptide 

 

For custom monoclonal antibody development/production service from peptide, customer provides the sequence information of the protein (Ref. The Gene Accession number from NCBI gives details of antigen information). We go via the peptide route to produce antibody for you. This is achieved by selecting the epitopic region (possible immunogenic sites) from the sequence and immunizing the mice with the peptide conjugated to a carrier molecule. 

C. Complete Package for Custom Monoclonal Antibody Development/Production Service-Partial recombinant protein 

For custom monoclonal antibody development service from partial recombinat protein, customer provides the sequence information of the protein (Ref. The Gene Accession number from NCBI gives details of antigen information). Custom monoclonal antibody development service includes all the features of partial package for custom polyclonal service apart from the following.

1. Identification of target antigen

2. Gene Synthesis and Partial Recombinant protein purification (Upto 200 amino acid) 

There are four phases for each custom monoclonal antibody development project. Each phase has its own duration and pricing structure. Movement from one phase to the other would be discussed with the customer and depending on the progress, decision would be taken. We shall do our best to get things done faster. However, there are multiple factors those influence the progress while meeting the dead line and that has to be sorted out amicably. Customer can start a project for a particular phase with a single purchase order (PO) and move on to the next phase with a separate PO or send order including all the features at a time.

 

 

 

Phase I: Antisera Development in Mouse
Duration: 6-9 weeks 

We check the purity of the customer supplied protein in SDS-PAGE, to ensure the integrity of the protein before initiating the project.

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Choice of animal is Balb/c mice. We introduce 4 mice for each antigen. Quantity of immunogen required is minimum 5 mg. We prefer the customer to supply it in a single batch. However, it can also be accepted in two batches if the protein is difficult to express or purify. We have a standard immunization schedule specific for peptide antigen and for native protein/recombinant protein antigen. However, customer can feel free to advise us the immunization schedule depending on the antigen they supply. At the end of immunization the animals are bled and checked for the specific titer by an indirect ELISA using free immunogen as antigen and if desired, Western blot can also be done. At the end of this stage, we would send at least 20 µl of test sera sample along with the pre immune sera to the customer for testing at their end. We give guarantee of ELISA positive sera only on antigen supplied by us. For customer supplied antigen, we can attempt a few more boosters if the sera are negative or non specific in the test system, before calling it off. After results are available and acceptable to the customer, we would proceed to Phase II.

 

Phase II: Fusion
Duration: 8-10 weeks 

http://abgenex.com/images/content/Ph-2.jpgAfter customer checks the specificity and affinity of the antibody in mouse sera, we decide to go for generation of mAb using PEG fusion technology (The mouse to be fused would be specified by the customer). Splenocytes are fused with suitable myeloma partner, and hybridoma clones are raised. Growing colonies are screened for their Ab producing capacity and detected by ELISA and if desired western blot (depending on the availability of the testing materials) can also be done free of charge. Strong ELISA positive clones are selected, expanded and frozen. At this stage, a minimum of 5-10 ml of culture supernatant from positive clones is also supplied to customer for validating antibody in desirable application system. If the customer wants to wind up the project at this stage, then we supply hybridoma cells (ELISA positive) to customer. However, if the customer wants the project to be continued, we send everything at the completion of each project. Customer must be aware that we can guarantee only ELISA positive clones.

 

Phase III: Sub-cloning and isotyping
Duration: 3-5 weeks 

http://abgenex.com/images/content/Ph-3.jpgAfter thorough investigation when the validity of the antibody application is confirmed, repeated attempts would be made to generate stable single cell clone stage through limiting dilution. Sub-cloning would be continued until we get a stable clone that produces antibody continuously and specifically. At this stage, the culture supernatants from two sub-clones would be sent to customer for verification. We would also do the isotyping of the clones at this stage and preferably select IgG isotypes, unless the customer wants otherwise. We will send the live cells at room temperature to avoid any damage due to the frozen cells during dry ice shipment. We can validate the mAb for other applications; for example, FACS, IP, IHC and IF at extra charge (see Additional Services).

 

Phase-IV: Large scale production of mAb and Purification
(Min. ~2-3 mg of Purified Antibody will be supplied)
Duration: 2-4 weeks 

http://abgenex.com/images/content/Ph-4.jpgWe select the clone suggested by customer. We scale up the mAb production using spinner flasks in continuous culture method. Then we purify the mAb from culture supernatant using specialized matrix column. We provide the purified antibody along with QC data.

 

 

Recombinant Protein Production

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Contact us before starting your next custom recombinant protein production/expression/purification project, we will work with you to determine your requirements and prepare a detailed proposal.

Salient features of recombinant protein production/expression/purification service

  • ~2-5 mg of the purified protein will be supplied.
  • Q.C. of the purified protein will be carried out by SDS-PAGE and WB by anti Anti-His antibody.
  • We also produce Functional Grade Recombinant Protein.

Procedure for Recombinant protein production/expression/purification

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Stable Cell Lines Development

We provide a full range of custom services for the life science & biomedical industry in the field of drug discovery. The stably-transfected cell lines are the best available solution for a wide range of applications, such as functional studies, drug discovery assays or the production of recombinant proteins. In contrast to transient expression, stable expression allows long term, as well as defined and reproducible, expression of the gene of interest. With state of art laboratory facility and world-class expertise, we offer a comprehensive service for the development of stable cell line.

All projects begin with a project evaluation in which we discuss and review your requirements. This includes target evaluation and applications. Then we prepare a project proposal and quotation based on your needs. This will address all costs as well as the scope of work to be completed and the estimated timeline of the project. 

Our Custom Service Manager is available to consult with you at any time, and will provide you with updates in regular basis.

Procedure for development of Stable Cell Lines

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Peptide Synthesis

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Linear Peptide Synthesis

In accordance with customer demand BioString Inc. can supply high - quality Linear peptides.

With mature peptide synthesis and purification methods, especially SPPS method and liquid phase synthesis method, we provide customers with high quality peptides:

  • Purity: crude ,70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% .
  • Desalination and Salt transfer : according to customers’ requirements, we can do TFA salt treatment, can also convert to acetate.
  • Delivery time: within 30 amino acids, about 2-3 weeks, urgent order, 1-2 weeks.
  • Quality control: providing MS, HPLC and COA reports documents of each peptide with delivery free.
  • After-sales service:  problems submitted in 1-2 weeks, we provide free-issue retest ,and refund for
  • Unqualified products. Ineligible goods retesting in 1-3 month free,and store samples1-2 weeks.

 

Long Peptide Synthesis

BioString provides long peptide synthesis service within the length of 150 amino acids. When peptide sequence is too long, it often appears that lack of residues, difficulty of condensation of amino acids and so on in the process of peptide synthesis. We have developed three schemes which effectively improve reaction on base of these problems.

 

  • Microwave synthesis: for some amino acids hard to condensate in the process of synthesis, we used microwave synthesiswhich is a significant effect method and greatly reduced the reaction time.
  • Fragment synthesis method: when it is difficult to synthesize certain peptides with conventional synthesis method, we will alsosynthesize a few amino acids as a fragment, then as a whole condensate to the peptide chain. This method can solve manyproblems existing in the synthesis.
  • 3.Hydrazine synthesis: A synthesis method to connect the peptides through forming amide bond with selective chemical reaction between N-terminal Cys peptide and C-terminal peptide acyl hydrazine in the solid phase synthesis.According to the position of Cys in peptide, we divide the entirepeptide chain into a plurality of sequences to synthesize separately, eventually to obtain the target peptide using liquid phase condensation reaction,which significantly improvesthe purity of the final product, widely used in the synthesis of long peptide containing Cys  .
    With mature peptide synthesis technology, Bankpeptide can fast design effective synthesis scheme and provide better service according to the customers requests, which is our constant pursuit.

 

Peptide Modification

 

BioString can provides a wide rang of peptides with various modifications according to customers’ requires!

 

  • Phosphorylation of Ser, Tyr and Thr modified peptides: we provide a single phosphorylation and multi phosphorylated peptide services. Now we have been able to provide four phosphorylated sites modified peptides.
  • Fluorescent labeling ,such as 5 (6) -FAM, FITC, Cy5, Rhodamine B, PNA, EDNAS/dabcyl etc.

Which is our representative technology and already quite mature.

  • Biotin, Lys (biotin) : Biotin is one part of the vitamin B2, and biotin, Lys (biotin) modified peptides are also often purchased by customers. We offer a biotin modified peptide that already has a success rate of nearly 100%.
  • Disulfide Bridge: disulfide bridge bond plays an important role in the stability of protein structure.we have been able to provide customers up to four disulfide bridges in one peptide .
  • Isotope labeling (C13, N15 labeling): isotope labeled peptides are mainly used in medical and biological fields at higher prices, In order to meet the needs of customers, we accept micrograms isotope labeling peptide customization.
  • Other special amino acid modification: for example, D amino acids, amino acid derivatives, aliphatic carboxylic acidsand so on, which all in our acceptable customization.

 

Confidentiality
Confidential Agreement will be signed upon request by customer to protect sensitive information such as peptide sequence information.

Custom Gene Synthesis

BioString Inc. can synthesize genes of any length and complexity to suit your needs. We can synthesize and clone any DNA sequences (even complex, GC-rich, repeated, or long genes) into prokaryotic, eukaryotic or viral vectors. Genes up to 3,000 bp are normally synthesized in 1-2 weeks!

Features & Benefits

  • Free codon optimization upon request.
  • Accept genes over 40-kb in length.
  • Flexible cloning choices: standard cloning, custom cloning and GB Seamless Cloning.
  • Free subcloning into one of our standard vectors.
  • Subcloning into any vector of our choice.

As fast as 5 Business Days, on average 2 weeks turn around time.

100% sequence-guaranteed synthetic genes of the highest quality.

Guarantee: BioString Inc. will guarantee appropriate genes (see Terms & Conditions) to ship from BioString Inc within the above turnaround times once an order has been approved. Appropriate genes that fail to meet the business day turnaround time posted will be credited with half the gene cost on your BioString Inc account (could be used for your further orders).

Terms & Conditions: Our turnaround guarantee is not valid for orders requiring custom cloning, genes with high GC/AT, stretches of homopolymers, low copy vectors, extensive repeats or genes longer than 3kb. Turnaround times can be affected if a gene is toxic to E.coli at propagation. Orders placed before 5:00pm EST (on USA business days) will be considered ordered on the same day.

Service Procedure

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Standard Deliverables

- 4-5 ug Lyophilized DNA in tubes or plates
- Plasmid map (Electronic)
- Sequence chromatograms covering your gene (electronic)
- Certificate of Analysis (COA)
Special notice: We promise not to release or use customer's gene sequence or other related information in any forms. We will destroy all primers, plasmids and strains after the products have been delivered for one month.

High-throughput DNA Sequencing and Genome Analytics Solutions

BioString Inc. has been partnering with academic and translational scientists by delivering next generation DNA sequencing and genome analytics services. We assist in attaining full potential of genomics by creatively exploring, analyzing, and inferring large volumes of genomic data. Our solutions augment the discovery process and empower scientists while saving precious time and research costs.

Our Services and Solutions

Genome Sequencing and Analysis

  • De-novo Genome Assembly
  • WG Re-sequencing Analysis
  • Exome Analysis
  • Targeted Re-sequencing Analysis
  • SNP Association Analysis
  • CNV/LOH Analysis
  • 16S rRNA Based Metagenome Analysis
  • Shotgun Metagenome Analysis

Transcriptome Sequencing and Analysis

  • RNA-seq Analysis
  • miRNA-seq Analysis
  • Degradaome Analysis
  • RIP-Analysis
  • Microarray-expression Analysis

Epigenome Sequencing and Analysis

  • Bisulfite-seq Analysis
  • ChIP-seq Analysis
  • MeDIP-seq Analysis
  • Microarray-methylation Analysis

LIQUID CHROMATOGRAPHY AND TANDEM MASS SPECTROMETRY ( LC-MS/MS )

Biostring Inc. offers mass spectrometry services for protein identification, protein quantitation and post translational modification (PTM) mapping. Biostring Inc. utilizes high performance nano liquid chromatography (LC) and Tandem mass spectrometry (MS/MS) technologies to achieve unsurpassed resolution and identification accuracy. We can tailor the LC phase to analyze proteins from a simple gel plug to a complex sample like cell lysate or plasma. To increase coverage of protein identification when dealing with a complex sample like serum, plasma, whole cell lysate and tissue homogenate we utilize Multi-Dimensional Protein Identification Technology (MudPIT) to reduce sample complexity prior to LC-MS/MS.

Quantitative protein analysis is another significant area offered along with simultaneous protein identification. We can do both the label-free approach (spectral counting or ion intensity measurements) and the labeled approach using Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) and Tandem Mass Tags (TMT). We have successfully finished multiple projects utilizing either the label-free approach or iTRAQ and TMT based biomarker identifications and we provide results either as relative abundance (iTRAQ or TMT ratios) or absolute quantities (pmol, pg, ug or fg).

Comprehensive identification of post translational modifications (PTM) is the third most sought after service at Biostring Inc.. We offer simple PTM analysis utilizing a multistage activation approach. For extensive PTM analysis we use either immobilized metal affinity chromatography (IMAC) or Titanium dioxide (TiO2) for phosphopeptide enrichment. For very complex samples, Biostring Inc. starts with Strong Cation Exchange (SCX) fractionation followed by IMAC or TiO2 phosphoenrichment. For lysine rich proteins or proteins without/minimal trypsin cleavable sites, we utilize a variety of nontraditional enzymes like LysC, ArgC, AspN and GluC for good coverage and PTM results.

To ensure that proteins are sufficiently digested into peptides prior to LC-MS/MS, ITSI-Biosciences utilizes the proprietary ProDM kit to accurately calculate the percentage of proteins digested into peptides.

Our team can perform global proteomics, protein expression profiling, comparative proteomics and targeted proteomics for biomarker discovery and monitoring. Most biological and clinical samples can be analyzed directly or after electrophoresis (e.g. 2D-DIGE, SDS-PAGE) and candidate proteins can be identified by nano LC/MS/MS after in-gel or in-solution digestion.

Our Protein Identification and proteomics services include experimental design, sample preparation, tandem mass spectrometry analysis using nano LC-MS/MS, database search, identified protein annotation and report preparation with suggested next steps.

We offer the following services:

NAME

SERVICE DESCRIPTION

UNIT COST

Sample Preparation 1

Homogenization, protein quantitation using ToPA Bradford protein assay kit.

Per sample

Sample preparation 2

Includes, precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA.

Per sample

Sample preparation 3

Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Column kit.

Per sample

Sample preparation 4

Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Solvent kit.

Per sample

Sample preparation 5

Depletion IgY Column Depletion of 14 abundant proteins from serum / plasma using IgY column.

Per sample

Sample preparation 6

Phospho-protein/ peptide enrichment using TiO2 or IMAC technology.

Per sample

Sample preparation 7

In-gel digestion. Digestion of proteins in a gel band/spot using trypsin. The service includes a) gel de-staining – coomassie or silver stain b) alkylation and reduction    c) trypsin digestion d) peptide extraction from the gels for mass spectrometry analysis.

Per sample

Sample preparation 8

In-solution digestion. Digestion of proteins in solution using trypsin. The service includes; a) determining protein concentration (if possible), b) alkylation and reduction c) digestion d) peptide extraction for mass spectrometry analysis.

Per sample

Sample preparation 9

Urine protein isolation and concentration. Service includes Isolation, partial purification, and concentration of urine proteins using ITSI Urine Protein Isolation kit.

Per sample

Sample preparation 10

Zip tip cleanup of in-gel or in-solution digested samples….

Per sample

Sample preparation 11

Any other sample preparation.

Per sample

Flash Proteomics Service (FPS) by tandem mass spectrometry

The Flash Proteomics Service involves the use of validated pre-fractionation protocols and high performance tandem mass spectrometers to rapidly generate a global qualitative protein signature of a moderately-complex specimen. With FPS the run time is 30 minutes or less and most results will be ready in about 48h.Samples including fractions from HPLC, pre-fractionated samples such as protein lysates, serum, plasma and culture media can be screened. In many cases,results will be    ready in 48 hours. This service is ideal for preliminary data generation, to support grant applications, biomarker research, search for contaminants in samples, verification of the integrity and purity of samples, characterization of antibodies and recombinant proteins.

The standard FPS service includes:

  • Sample preparation,
  • Nano-LC/MS/MS.
  • Database search and identified protein annotation.

Per sample

LC60-MS/MS

Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of pure protein samples. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation – 60 min run.

Per sample

LC90-MS/MS

Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of slightly complex samples, including proteins isolated from gel plugs and gel bands. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation- 90 min run.

Per sample

LC120-MS/MS

Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of moderately complex samples. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation – 120 min run.

Per sample

LC240-MS/MS

Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of very complex samples. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation – 240 min run.

Per sample

LC-MS/MS-PTM

Peptide sequencing and Post Translational Modification (PTM) Mapping of purified or partially purified proteins by Mass Spectrometry. Services include

  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search
  • PTM Mapping and
  • Identified protein annotation.

Per sample

MudPIT Proteomics.

Multi-dimensional Protein Identification Technology and peptide sequencing. Services include

  • Off line SCX fractionation into multiple fractions,
  • Online Reverse Phase (RP) liquid chromatography (LC) – tandem mass spectrometry analysis using nano- LC/MS/MS,
  • Database search and
  • Identified full protein annotation.

Per sample

iTRAQ based Proteomics.

Quantitative proteomics using Isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Labeling with 2-8 tags can be used. iTRAQ Labeling is a non-gel based protein quantitation technique using 5 – 100 µg of protein. 2 to 8 samples can be compared in a single experiment. Services include

  • In-solution digestion
  • iTRAQ labeling
  • SCX cleanup,
  • Tandem mass spectrometry analysis using nano- LC/MS/MS,
  • Database search and
  • Identified full protein annotation.

2-8 samples

iTRAQ-MudPIT Proteomics.

iTRAQ plus MudPIT. Service includes

  • Multi-dimensional offline fractionation of samples into 2-20 fractions,
  • LC-MS/MS analysis of all fractions,
  • Database search and annotation of identified proteins.

Per sample

TMT based Proteomics.

Quantitative proteomics using Tandem Mass Tags (TMT). Labeling with 2-8 tags can be used. TMT Labeling is a non-gel based protein quantitation technique using 5 – 100 µg of protein. 2 to 8 samples can be compared in a single experiment. Services include

  • In-solution digestion
  • TMT labeling
  • SCX cleanup,
  • Tandem mass spectrometry analysis using nano- LC/MS/MS,
  • Database search and
  • Identified full protein annotation.

2-8 sample

TMT-MudPIT Proteomics.

TMT plus MudPIT. Service includes

  • Multi-dimensional offline fractionation of samples into 2-20 fractions,
  • LC-MS/MS analysis of all fractions,
  • Database search and annotation of identified proteins.

Per sample

xMAP analysis

Quantitative protein analysis using Luminex xMAP technology. The level of up to 100 proteins can be measured simultaneously in 50ul of serum, plasma, tissue lysate and other body fluids.

1-100 samples

Metabolomics – PMP

Primary Metabolism Profiling (PMP): This analysis targets carbohydrates and sugar phosphates, amino acids, hydroxyl acids, free fatty acids, purines, pyrimidines, aromatics, exposome-derived chemicals.

Per Sample (minimum 12 samples)

Metabolomics – CLP

Complex Lipids Profiling (CLP): This analysis will target Ceramides, Sphingomyelins, Cholesteryl esters, Oxysterols, Lyso- and phospholipids, mono-, di- and triacylglycerols, galactosyl- and glucuronyllipids.

Per Sample (minimum 12 samples)

Metabolomics – BAP

Biogenic Amines Profiling (BAP): This analysis will target acylcarnitines, TMAO, cholines, betaines, SAM, SAH, nucleotides and nucleosides, methylated and acetylated amines, di- and oligopeptides.

Per Sample (minimum 12 samples)

Metabolomics –TMA

Targeted Metabolite Analyses (TMA): This analysis measures bile acids, steroids, eicososanoids / oxylipins and, very short chain fatty acids using liquid chromatography-QTRAP or gas chromatography-quadrupole mass spectrometry

Per Sample (minimum 12 samples)

Database Search – Protein Identification

Database search for protein identification. The service includes

  • Downloading the most recent database files from NCBI or other relevant sources
  • Database search and
  • Identified protein annotation.

Per search

Database Search – Protein Identification and PTM Mapping

Database search for protein identification and post translational modification mapping. The service includes

  • Downloading the most recent database files from NCBI or other relevant sources
  • Database search and
  • Identified protein annotation.

Per search

Gene Ontology (GO) Annotation

Annotation of proteins using GO classification for biological process, molecular function and cellular component.

Per search

Manual Spectrum Interpretation

Manual Interpretation of mass spectrum

Per hour (Minimum one hour)

 

 

WELLNESS

In wellness test we combine the genetic results with the lifestyle data and create a personalised, safe and balanced nutrition guide with the optimal caloric load and macronutrient ratios.

Wellness is categorized into following:

Diet:

  • Diet is all the food consumed by a person or the other organism.
  • Therefore, Vitamins, macro-nutrients, and food intolerance included under this section.
  • Armed with information that is specific to the genetic profile, healthcare professionals will be able to develop the best dietry plan to meet one's nutrition & fitness goals.
  • Adhering to those recommendations will enable an individual's nutritional status and prevent nutrition-related chronic diseases.

Weight:

  • Weight management describs the techniques and underlying physiological processes that contribute to a person's ability to attain and maintain a certain weight.
  • Genetics play a role in determining the weight.
  • Environment, lifestyle and healthy choices also contribute a great deal in weight.
  • Genes make difference in the type of weight-loss strategies that may work best for the individual.

Fitness:

  • By Analysing the genes, best suited physical activity is recommended. 

Detox:

  • Detoxification means cleansing the blood.
  • We report genes for detoxification by fat soluble, water soluble & oxidative stress.
  • The test shows what lifestyle is ideal to support optimal enzyme interaction and protect against the development of various types of cancer.

TWO-DIMENSIONAL DIFFERENCE IN-GEL ELECTROPHORESIS (2D-DIGE)

Biostring Inc. utilizes validated sample preparation kits/protocols, robotic spot pickers/digesters, DeCyder difference-in-gel analysis software and advanced mass spectrometers to offer an integrated 2D-DIGE – MS/MS proteomics solution. Proteomic analysis usually starts from sample preparation, to analyses by mass spectrometry (LC-MS/MS) and protein identification and annotation. Data obtained after 2D-DIGE analysis may include protein identity, pI, molecular weight and post translational modifications.

Biostring Inc. has provided 2D-DIGE analysis and proteomics services to NIH, industry, biotechnology companies, pharmaceutical companies, Research Institutes and Universities. Our more than 15 years of experience with 2D-DIGE analysis, proteomics, mass spectrometry and protein expression profiling enables us to offer reproducible picture perfect gels at a competitive price and guaranteed faster turnaround time. We have also developed a variety of complementary 2D-DIGE kits to streamline and standardize protein isolation, protein quantitation, protein qualification and the 2D-DIGE analysis step, thereby reducing the overall time and cost of performing 2D-DIGE analysis.

2D-DIGE offers all the advantages of the classical 2D-PAGE, and most importantly overcomes the inherent disadvantage of gel-to-gel variation and reproducibility problem inherent with the traditional 2D-PAGE approach. 2D-DIGE is presently the most versatile gel-based approach for comparative and semi-quantitative proteomics. Distinct fluorescent tags e.g. Cy 3, 5 and 2 are used to label protein samples and a universal internal standard prior to 1st/2nd dimension electrophoresis. An automated software program is used to detect, quantify and annotate differentially expressed proteins. 2D-DIGE analysis can be applied to protein expression profiling and comparative proteomics by using DeCyder software to identify proteins with statistically-significant difference in expression. This provides unique opportunities for researches in fields including cancer, neuroscience, vision, cardiovascular, Alzheimer, Parkinson, etc, to find important proteins associated with the condition. 2D-DIGE – LC/MS/MS analysis can also be applied to identify post translationally modified proteins (PTM) that are important in cell signaling and protein trafficking.

 

Our 2D-DIGE based comparative proteomics services includes, experimental design, Sample preparation to isolate total proteins using ToPI-DIGE protein isolation Kit, pre-fractionation, quantitation and qualification of proteins, 2D-DIGE analysis, scanning of gels using a Typhoon DIGE-enabled digital scanner, Biological Variation Analysis (BVA) of spots detected to identify differentially expressed proteins with statistically significant difference, robotic spot picking of candidate proteins, robotic in-gel digestion of candidate proteins with trypsin or another enzyme. The enzyme digested proteins are identified by Tandem Mass Spectrometry and annotated.

We offer the following services:

NAME DESCRIPTION UNIT COST
Sample Preparation 1 Homogenization, protein quantitation using ToPA Bradford protein assay kit. Per sample
Sample Preparation 2 Includes, precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA kits. Per sample
Sample Preparation 3 Depletion of abundant protein e.g. bovine or human serum albumin using ASK-Column kit. Per sample
Sample Preparation 4 Depletion of abundant protein e.g. bovine or human serum albumin using ASK-Solvent kit. Per sample
Sample Preparation 5 Depletion IgY Column Depletion of 14 abundant proteins from serum /plasma using IgY column. Per sample
Sample Preparation 9 Urine protein isolation and concentration. Service includes Isolation, partial purification, and concentration of urine proteins using Urine Protein Isolation kit. Per sample
2D-DIGE Two- Dimensional Difference in-Gel Electrophoresis (2D- DIGE). Quantitative and comparative proteomics using 2D-DIGE. Services include:
  • Experimental design,
  • Sample preparation to isolate total proteins using ToPI- DIGE Protein Isolation Kit,
  • Fractionation, quantitation and qualification of proteins,
  • 2D-DIGE analysis using Cy2, Cy3 and Cy5, scanning of gels using a DIGE- enabled digital scanner and
  • Biological variation analysis of gel images to identify differentially expressed proteins with statistically significant difference.
2 – 10 Samples
Robotic spot picking Selection and picking of fluorescent labels protein spots from 2D-DIGE gels using fully automated ETTAN spot picking robot. 1-96 spots
Robotic spot digestion In gel digestion of protein spots picked from 2D-DIGE gels using a fully automated ETTAN spot digestion robot. 1-96 spots
LC60-MS/MS Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of pure protein samples. Services include:
  • Tandem mass spectrometry analysis using nano-LC/MS/MS,
  • Database search and
  • Identified protein annotation – 60 min run.
Per sample

QUANTITATIVE MULTIPLEXED PROTEIN ANALYSIS USING THE LUMINEX XMAP

We utilize the Luminex xMAP system to provide multiplex protein biomarker monitoring, validation and screening. Multiple target proteins can be monitored simultaneously in the same samples. Kits developed by Luminex strategic partners that have received 510(k) clearance from the FDA can be used on the Luminex system for diagnostic purposes. Custom assays can be developed using antibodies supplied by the client or off the shelf assays can be obtained from strategic partners for most biomedical research applications.

Most biological and clinical samples can be analyzed, including, tissue homogenate, cell lysate, whole blood, serum, plasma, purified proteins and formalin-fixed paraffin embedded samples. Partial list of assays available: cancer markers, cardiac markers, cellular signaling, cytokines, chemokines, and growth factors, endocrine, isotyping, matrix metalloproteinases, metabolic markers, neurobiology, and transcription factors/nuclear receptors.

Why xMAP technology?

  • Multiplexing: Up to 100 bioassays per well; including controls.
  • Parallel analysis: simultaneous quantitation of multiple proteins in the same sample.
  • Sample amount: less than 50ul for up to 100 data points.
  • Reproducibility: provides consistent results and allow for easy quality control.
  • Accuracy: Quantitative results over a 3 to 4 log dynamic range, with strong concordance to ELISA and mass spectrometry.

We offer the following services based on the xMAP technology:

NAME DESCRIPTION UNIT COST
Sample Preparation 1 Homogenization, protein quantitation using ToPA Bradford protein assay kit. Per sample
Sample Preparation 2 Includes precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA kits. Per sample
Sample Preparation 3 Depletion of abundant protein e.g. bovine or human serum albumin using ASK-Column kit. Per sample
Sample Preparation 4 Depletion of abundant protein e.g. bovine or human serum albumin using ASK-Solvent kit. Per sample
Sample Preparation 5 Depletion IgY Column Depletion of 14 abundant proteins from serum / plasma using IgY column. Per sample
xMAP analysis

Quantitative protein analysis using Luminex xMAP technology. The level of up to 100 proteins can be measured simultaneously in 50ul of serum, plasma, tissue lysate and other body fluids.

1-100 samples
Assay Development for xMAP analysis of Proteins This service involves development assays to target proteins(s) of interest. Per target

QUANTITATIVE MULTIPLEXED RNA ANALYSIS

Biostring Inc. utilizes the Panomics QuantiGene Plex Branched-DNA and Luminex xMAP technologies (QuantiGene-xMAP) to offer multiplexed RNA analysis. Our service includes experimental design, Sample preparation, use of commercially available or in-house developed probes to analyze samples and full statistical data analysis.

Multiple RNA targets can be monitored simultaneously in the same sample. The sensitive and reproducible QuantiGene SinglePlex and MultiPlex technology and Reagent systems has higher sensitivity than most micro arrays and this technology does not require RNA isolation, target amplification by PCR and reverse-transcription.

QuantiGene-xMAP can be applied for research in the following areas: cancer, cardiac disease, cellular signaling, cytokines, chemokines, growth factors, endocrine, matrix metalloproteinases, metabolic disease, neurobiology, transcription factors and nuclear Receptors. Most biological and clinical samples can be analyzed, including, tissue homogenate, cell lysate, whole blood, purified RNA, serum, plasma, and formalin-fixed paraffin embedded samples.

Partial list of assays available: 1L6R, ACTB, BAD, BAK1 (BAK), BCL2, BCL2L1 (BCL-XL), CDKN1A (CDKN1), CFLAR (CFLIP), CSF2, GAPD, IFN-gamma, IL-1 beta, IL-10, IL-2, IL-6, IL-8, NFKB2, NFKBIA (NFKIA), NKFB1, PPIB, Ptk2B (RAFTK), RELA, RELB, TNF, TNFAIP3 (A20), TNFRSF6 (FAS), TNFSF6 (FASL), and VEGF.

Why QuantiGene – xMAP technology?

  • Multiplexing: Up to 100 bioassays per well; including controls.
  • Parallel analysis: Simultaneous quantitation of multiple RNAs in the same sample.
  • Sample amount: Less than 50ul for up to 100 data points.
  • Reproducibility: Provides consistent results and allow for easy quality control.
  • Accuracy: Quantitative results over a 3 to 4 log dynamic range, with strong concordance to micro array and real time PCR.

We offer the following services:

NAME DESCRIPTION UNIT COST
Sample Preparation for QuantiGene -xMAP Preparation of samples for SP1QGX. This service may include homogenization, filtration, fractionation, centrifugation or dilution of samples prior to analysis. Per sample
QuantiGene – xMAP analysis of RNA Quantitative multiplexed RNA analysis using Panomics QuantGene Plex and Luminex xMAP technology. The level of up to 100 RNA targets can be measured simultaneously in 50ul of serum, plasma, tissue lysate and other body fluids. 1-100 samples
Assay Development for QuantiGene – xMAP analysis of RNA This service involves development assays to target RNA(s) of interest. Per target